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これまでの業績

年代順に並べてみました。。*印はCorresponding authorです。最後に総説があります。

2011年以降

  1. Mizuno R., KamiokaY., Kabashima K., Imajo M., Sumiyama K., Nakasho E., Ito T., Hamazaki Y., Okuchi Y., Sakai Y., Kiyokawa E., Matsuda M.: In vivo FRET imaging of activities of PKA and ERK during neutrophil recruitment to inflamed intestines. J Exp Med. 211(6):1123-36, 2014.
  2. Kumagai Y., Naoki Y., Nakasho E., Kamioka, Y., Kiyokawa E, Matsuda M.: Heterogeneity in ERK activity as visualized by in vivo FRET imaging of mammary tumor cells developed in MMTV-Neu mice. Oncogene: in press
  3. Mori Y., Yagi S., Sakurai A.,Matsuda M., Kiyokawa E*.: Insufficient ability of Rac1b to perturb cystogenesis. Small GTPases:4(1) 9-15, 2013
  4. Yagi S., Matsuda M., Kiyokawa E*.: Chimaerin suppresses Rac1 activation at the apical membrane to maintain the cyst structure. PLOS ONE., 7(12), e52258, 2012.
  5. Sakurai A., Matsuda M., Kiyokawa E*.: Activated Ras protein accelerates cell cycle progression to perturb MDCK cystogenesis. J Biol Chem, 287(38), 31703-31711, 2012.
  6. Kamioka Y., Sumiyama K., Sakai Y., Mizuno R., Hirata E., Kiyokawa E., Matsuda M.:Live Imaging of Protein Kinase Activities in Transgenic Mice Expressing FRET Biosensors. Cell Struc. & Func., 37(1), 65-73, 2012
  7. Yagi S., Matsuda M., Kiyokawa E*.: Suppression of Rac1 Activity at the Apical Membrane of MDCK cells is Essential for Cyst Structure Maintenance. EMBO Rep: 13(3), 237-243, 2012.
  8. Kumagai Y., Kamioka, Y., Yagi S., Matsuda M., Kiyokawa E*.: A genetically encoded Forster resonance energy transfer (FRET) biosensor for two-photon excitation microscopy. Anal. Biochem. 413(2), 192-199, 2011.

2001年～2010年

  1. Nishioka T., Frohman, MA., Matsuda M., Kiyokawa E*.: Heterogeneity of Phosphatidic Acid Levels and Distribution at the Plasma Membrane in Living Cells as Visualized by a Forster Resonance Energy Transfer (FRET) Biosensor. J Biol Chem: 285 (46): 35979-35987, 2010.
  2. Yoshiki S., Udagawa R., Aoki K., Kamioka Y., Kiyokawa E., Matsuda M.: Ras and Calcium signaling pathways converge at Raf1 via the Shoc2 scaffold protein. Mol Biol Cell: 21 (6): 1088-1096, 2010.
  3. Matsunaga-Udagawa R., Fujita Y., Yoshiki S., Terai K., Kamioka Y., Kiyokawa E., Yugi K., Aoki K., Matsuda M.: The scaffold protein Shoc2/SUR-8 accelerates the interaction of Ras and Raf. J Biol Chem.: 285(10): 7818-7826, 2010.
  4. Tachibana M., Kiyokawa E*., Hara S., Iemura S., Natsume T., Manabe T., Matsuda M.: Ankyrin repeat domain 28 (ANKRD28), a novel binding partner of DOCK180, promotes cell migration by regulating focal adhesion formation. Exp. Cell Res. 315(5): 863-876, 2009.
  5. Itoh RE., Kiyokawa E., Aoki,K., Nishioka T., Akiyama T., Matsuda M.: Phosphorylation and activation of a Rac1/Cdc42 guanine nucleotide exchange factor Asef in A431 cells stimulated by epidermal growth factor. J. Cell Sci. 121 (16): 2635-2642, 2008.
  6. Nishioka T., Aoki K., Hikake K., Yoshizaki H., Kiyokawa E*., Matsuda M.: Rapid turnover rate of phosphoinositides at the front of migrating MDCK cells. Mol Biol Cell 19(10): 4213-4223, 2008.
  7. Hara S., Kiyokawa E*., Iemura S., Natsume T., Wassmer T., Cullen PJ., Hiai H., Matsuda M.: The DHR1 domain of DOCK180 binds to SNX5 and regulates cation-independent mannose 6-phosphate receptor transport. Mol Biol Cell 19(9): 3823-3835, 2008.
  8. Omi N., Kiyokawa E., Matsuda M., Yamada S., Yamada K., Kinoshita K., Wang Y., Matsushima Y., Kawai J., Suzuki M., Hayashizaki Y., Hiai H.: Mutation of Dock5, a member of the guanine exchange factor Dock180 superfamily, in the rupture of lens cataract mouse. Exp Eye Res., 86(5):828-34, 2008.
  9. Takaya A., Kamio T., Masuda M., Mochizuki N., Sawa H., Sato M., Nagashima K., Mizutani A., Matsuno A., Kiyokawa E., Matsuda M.: R-Ras regulates exocytosis by Rgl2/Rlf-mediated activation of RalA on endosomes. Mol Biol Cell 18 (5): 1850-1860, 2007.
  10. Kiyokawa E*., Baba T., Otsuka N., Makino A., Ohno S., Kobayashi T*.: Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J Biol Chem. 280(25):24072-84, 2005.
  11. Kiyokawa E., Makino A., Ishii K., Otsuka N., Yamaji-Hasegawa A., Kobayashi T.: Recognition of sphingomyelin by lysenin and lysenin-related proteins, Biochemistry 43, 9766-9773, 2004
  12. Yamaji-Hasegawa A, Makino A, Baba T, Senoh Y, Kimura-Suda H, Sato SB, Terada N, Ohno S, Kiyokawa E, Umeda M, Kobayashi T.: Oligomerization and pore formation of a sphingomyelin-specific toxin, lysenin. J Biol Chem. 278(25):22762-70, 2003.
  13. Guyader M., Kiyokawa E., Abrami L., Turelli P., Trono D.: Role for human immunodeficiency virus type 1 membrane cholesterol in viral internalization. J Virol. 76(20):10356-64, 2002.
  14. Vilhardt F, Plastre O., Sawada M., Suzuki K., Wiznerowicz M., Kiyokawa E., Trono D., Krause KH.: The HIV-1 Nef protein and phagocyte NADPH oxidase activation. J Biol Chem. 277(44):42136-43, 2002
  15. Tobiume M., Tokunaga K., Kiyokawa E., Takahoko M., Mochizuki N., Tatsumi, M., Matsuda, M.: Requirement of nef for HIV-1 infectivity is biased by the expression levels of Env in the virus-producing cells and CD4 in the target cells. Arch Virol. 146(9), 1739-51, 2001.
  16. Kobayashi S., Shirai T., Kiyokawa E., Mochizuki N., Matsuda M., Fukui Y.: Membrane recruitment of DOCK180 by binding to PtdIns(3,4,5)P3. Biochem J. 354 (1):73-8, 2001.

2000年以前

  1. Wang JK., Kiyokawa E., (The first two equally contributed) Verdin E., Trono D.: The Nef protein of HIV-1 associates with raft and primes T cells for activation. Proc. Natl. Acad. Sci. USA 97(1), 394-399, 2000.
  2. Katayama H., Hashimoto Y., Kiyokawa E., Nakaya M., Sakamoto A., Machinami R., Kurata T., Mochizuki N., Matsuda M.: Epidermal growth factor-dependent dissociation of CrkII proto-oncogene product from the epidermal growth factor receptor in human glioma cells. Jpn. J. Cancer Res. 90(10), 1096-1103, 1999.
  3. Mochizuki N., Ohba Y., Kiyokawa E., Kurata T., Murakami T., Ozaki, T., Kitabatake A., Nagashima K., Matsuda M.: Activation of the ERK/MAPK pathway by an isoform of rap1GAP associated with Gi. Nature 400, 891-894, 1999.
  4. Hashimoto Y., Katayama H., Kiyokawa E., Ota S., Kurata T., Gotoh N., Otsuka N., Shibata M., Matsuda M.: Phosphorylation of CrkII adaptor protein at tyrosine 221 by epidermal growth factor receptor. J Biol Chem. 273 (27): 17186-17191, 1998
  5. Tokunaga K., Kiyokawa E., Nakaya M.,Otsuka N., Kojima A., Kurata T., Matsuda M.: Inhibition of Human Immunodeficiency Virus Type-1 Virion Entry by Dominant Negative Hck. J Virol. 72 (7): 6257-6259, 1998.
  6. Kiyokawa E., Hashimoto Y., Kobayashi S., Sugimura H., Kurata T., Matsuda M.: Activation of Rac1 by a Crk SH3-binding protein, DOCK180. Genes Dev. 12 (21): 3331-3336, 1998
  7. Kiyokawa E., Hashimoto Y., Sugimura H., Kurata T., Matsuda M.: Evidence that DOCK180 up-regulates signals from the Crk-p130Cas complex. J Biol Chem. 273 (38): 24479-24484, 1998.
  8. Xiang Y.-Y., Wang D.-Y., Suzuki M., Kiyokawa E., Igarashi H., Naito Y., Shen Q., Sugimura H.: Expression of High Mobility Group-1 mRNA in human gastrointestinal adenocarcinoma and corresponding noncancerous mucosa. Int J Cancer 74 (1), 1-6, 1997.
  9. Kasami M, Yoshida M, Isogaki J, Ogawa H, Shinmura K, Endo Y, Kiyokawa E, Naito Y, Arai T, Kimura T, Inoue R, Sugimura H.: Bilateral breast tumors, malignant phyllodes tumor and invasive lobular carcinoma in a 46,XX/46,XY mosaic female with family history of breast cancer. Pathol Int., 47(2-3):147-54, 1997.
  10. Takai S., Hasegawa H., Kiyokawa E., Yamada K., Kurata T., Matsuda M.: Chromosomal mapping of the gene encoding DOCK 180, a major Crk-binding protein, to 10q26.13-q26.3 by fluorescence in situ hybridization. Genomics 35 (2): 403-404, 1996.
  11. Hasegawa H., Kiyokawa E., Tanaka S., Nagashima K., Gotoh N., Kurata T., Matsuda M.: DOCK180, a major CRK-binding protein, alters cell morphology upon translocation to the membrane. Mol Cell Biol. 16 (4), 1770-1776, 1996.
  12. Sugimura H., Hamada GS., Suzuki I., Iwase T., Kiyokawa E., Kino I., Tsugane S.: CYP1A1 and CYP2E1 polymorphism and lung cancer, case-control study in Rio de Janeiro, Brazil. Pharmacogenetics., 5: S145-148. 1995.
  13. Hamada GS., Sugimura H., Suzuki I., Nagura K., Kiyokawa E., Iwase T., Tanaka M., Takahashi T, Watanabe S., Kino I., Tsugane S.: The heme-binding region polymorphism of cytochrome P450IA1 (CypIA1), rather than the RsaI polymorphism of IIE1 (CypIIE1), is associated with lung cancer in Rio de Janeiro. Cancer Epidemiol Biomarkers Prev. 4(1):63-67, 1995.
  14. Kiyokawa E., Takai S., Tanaka M., Iwase T., Suzuki M., Xiang YY., Naito Y, Yamada K., Sugimura H., Kino I.: Overexpression of ERK, an EPH family receptor protein tyrosine kinase, in various human tumors. Cancer Res. 54(14):3645-50, 1994.
  15. Xiang YY., Tanaka M., Suzuki M, Igarashi H, Kiyokawa E., Naito Y., Ohtawara Y, Shen Q., Sugimura H., Kino I.: Isolation of complementary DNA encoding K-cadherin, a novel rat cadherin preferentially expressed in fetal kidney and kidney carcinoma. Cancer Res. 54(11):3034-41, 1994.

総説・英文　

  1. Minato H., Kiyokawa E.: Activated K-RAS and its effect on morphological appearance. J Biochem, 2014, 156(3): 137-145, 2014.
  2. Kiyokawa E, Aoki K., Nakamura T, Matsuda M.: Spatiotemporal Regulation of Small GTPases as Revealed by Probes Based on the Principle of Förster Resonance Energy Transfer (FRET): Implications for Signaling and Pharmacology. Annu. Rev. Pharmacol. Toxicol., 51: 337-358, 2010.
  3. Kiyokawa E*., Matsuda M.: Regulation of focal adhesion and cell migration by ANKRD28-DOCK180 interaction. Cell Adh. Migr. 3 (3): 281-284, 2009. *Corresponding author
  4. Nakamura T., Kurokawa K., Kiyokawa E., Matsuda M.: Analysis of the Spatio-Temporal Activation of Rho GTPases using Raichu Probes. Methods Enzymol. 406: 315-332, 2006.
  5. Kiyokawa E., Hara S, Nakamura T, Matsuda M.: Fluorescence (Förster) resonance energy transfer imaging of oncogene activity in living cells. Cancer Sci. 97(1):8-15, 2006
  6. Kobayashi T., Yamaji-Hasegawa A. Kiyokawa E.: Lipid domains in the endocytic pathway. Sem. Cell Dev. Biol. 12(2), 173-82, 2001
  7. Kiyokawa E., Mochizuki N., Kurata T., Matsuda M.: Role of Crk Oncogene product in physiologic signaling. Crit Rev Oncogenesis 8 (4): 329-342, 1997.

総説・日本語　

  1. 相川あかね　清川悦子 「Rhoファミリー」生体の科学　66(5), 404-405, 2015
  2. 清川悦子 「FRETバイオセンサーによって明らかとなった上皮構造維持における低分子量Gタンパク質Rac1の役割」 生化学　84(11), 947-951, 2012
  3. 清川悦子 「脂質の局在を可視化するFRETバイオセンサー」 金沢医科大学雑誌　37(2), 59-65, 2012
  4. 清川悦子　松田道行　「細胞内シグナルのin vivoイメージング」 病理と臨床　30(7), 712-718, 2012
  5. 水野礼　清川悦子　松田道行　「FRETによる細胞シグナルの分子イメージング」最新医学　66巻（通巻841号）　特集「分子イメージングの最先端」 2376-2383, 2011.
  6. 清川悦子　上岡裕治　松田道行　「癌のFRETライブイメージング」Surgery Frontier 18(1) 2011.
  7. 清川悦子　八木俊輔　松田道行　「Small Gタンパク質活性化の時空間情報を取得する」細胞工学　28(12), 1229-1233, 2009.
  8. 清川悦子　松田道行　「腫瘍をめぐるQ&A　分子イメージングについて教えてください」Surgery Frontier 16(4)　98-100, 2009.
  9. 清川悦子　松田道行　「シグナル伝達カスケードの生細胞イメージング」　Annual Review 「免疫」 303-312, 2008
  10. 清川悦子　松田道行　「細胞内イメージング　（FRETなど）」　遺伝子医学MOOK　9号　「ますます広がる分子イメージング技術」, 120-125, 2008
  11. 原重雄　清川悦子　松田道行　「癌遺伝子情報伝達系イメージング」 病理と臨床　25(6), 560-565, 2007
  12. 清川悦子　「スフィンゴ脂質の自己組織化と細胞機能」　バイオニクス　2(3), 74-75, 2005
  13. 清川悦子　「HIV-1のアクセサリ分子NefとT細胞活性化」　生化学　72(11), 1337-1340, 2000.
  14. 清川悦子　松田道行　「Crk」 Bio Science 用語ライブラリー　癌遺伝子・癌抑制遺伝子　46-47, 1997